Officials said another 44 people were injured in the pile-up at the toll station which is close to Lanzhou, capital of Gansu.
China's state-run news agency says at least 14 people have been killed in a highway pile-up in the northwestern province of Gansu.
It's understood a tower-crane truck lost control before ploughing into as many as 31 cars waiting at the Lanzhou South Toll Station about 7.20pm local time.
31 vehicles were involved in the horror crash, and 10 of the 44 injured were in a serious condition as a result, China Daily reported.
The truck driver, who was only slightly injured in the crash, is under police guard and is being investigated.
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The driver, who had never driven on this expressway before, panicked and did not steer away from traffic when his brake failed, according to the authorities.
Investigation into the cause of the accident is underway.
The truck driver who lost control of the Board, crashed into the 31 vehicle.
The Gansu provincial authorities demanded an examination of unsafe sections of expressways across the province.
Last week, 15 people were killed after a bus veered off a bridge into China's Yangtze River.