Tottenham MP David Lammy and peer Lord Adonis were among the speakers while MP Luciana Berger led the march.
"Are you listening Jeremy Corbyn?", he shouted into a microphone.
She was speaking at a Jewish Labour Movement event at the Labour party conference in Liverpool.
Mr Corbyn told the Mirror in an interview today: "If this Government can't deliver Brexit then it must move over and have a General Election". "There is no such thing as a good Brexit because Brexit means trashing jobs, trashing Ireland, trashing the young. and our socialist values".
Labour's Jeremy Corbyn has so far resisted calls to back a "People's Vote", or new referendum on the decision to quit the EU.
There was also a short speech by Everton and England footballing hero Peter Reid, who urged the crowd to "keep the blue flag flying".
The prime minister has found herself politically boxed in by the European Union overseas and at home by both opponents of Brexit who want a second referendum and "hard Brexiteers" in her own party who want a clean break with the EU.
"We would vote it down if it didn't meet our tests in order to send the government, if it is still in office, straight back to the negotiating table and if there is a general election and we are in office we would go straight to the negotiating table".
May says she will hold her nerve in the talks, pressing the European Union to come up with an alternative proposal to her Chequers plan, named after the prime minister's country residence where a deal was hashed out with her top ministers in July. "The question I think that has to be answered now by the government, by the European Union leaders, is what room for movement is there, how do we move on from where we ended up last week?" "That's why I think we now need to take a stand - we the Labour Party and we the country".
"I'm very disappointed Jeremy is not here".
Accuser of Supreme Court nominee Kavanaugh agrees to testify to Senate committee
They include Brett Kavanaugh , Mark Judge (the sort-of accomplice), Patrick "PJ" Smyth and now Leland Ingham Keyser. Ford ... the way to depoliticize that and ensure that is with an outside counsel", they said.
Corbyn allowed that if the Labour conference voted for a new referendum he would "not walk away from it and I will act accordingly".
Corbyn appeared on the BB'C's Andrew Marr Show this morning and said he hoped there would be a general election soon.
He replied: "Let's see what comes out of conference".
He said: "I'm bound by the democracy of our party".
Ahead of the vote thousands joined a protest march organised by the People's Vote campaign group yesterday.
"There will be a clear vote in the conference".
"No chance am I going to sit by without saying something".
The comments revived memories of the party's battles of the 1980s, when then leader Neil Kinnock denounced "the grotesque chaos of a Labour council - a Labour council - hiring taxis to scuttle round a city handing out redundancy notices to its own workers".
"By ensuring businesses reserve a third of board seats for workers, we'll help transform our broken economic model", said Corbyn, who has shifted Labour towards the hard left since being elected party chief in 2015.
"If the people's party decide they want the people to have a final say on the deal, we have to respect the view of our members and we will go out and argue for it", he said.